Cylinder 2 misfire

[JT78]

Im getting cylinder 2 misfires after switching back from e85flex to 93 -Lund tune.I Changed plugs to Bosch Double Iridium gapped at .051 ,replaced coil and fuel injector on cylinder 2,cleaned MaF sensor,did crank relearn.No luck.Thinking maybe I got a bad cat ,intake runner problem,or maybe I need to put oem plugs back in .Shouldnt their be more cylinders misfiring then just cylinder 2 if it was a intake or cat problem and shouldn’t that throw a different code? Any help appreciated…2017gt

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[MyStang]

I was getting Misfire on Cylinder P0301 on E-85.
Sent a cold start data log to the tuner, he could see fuel trims moving around. Check for Vacuum leak. I had a loose-fitting vacuum line that could have been a potential leak. So far so good.